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An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells
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Chapitres
- 00:05Titre
- 01:45Plasmodium falciparum-infected Red Blood Cell (iRBC) Purification
- 02:35Exposure of Monocyte-derived Macrophages (MDM) to Plasmodium falciparum
- 03:40Infection of MDM with a Fully Replicative HIV-1
- 04:44Infection of MDM with Single Cycle Virus Encoding Luciferase
- 05:56Results: Exposure of P. falciparum to MDM Decreases their Susceptibility to HIV-1 Infection
- 07:16Conclusion
We have developed an in vitro malaria-HIV-1 co-infection model to study the impact of Plasmodium falciparum on the HIV-1 replicative cycle in human primary monocyte-derived macrophages. This versatile system can easily be adapted to other primary cell types susceptible to HIV-1 infection.
Tags
In Vitro Co-infection ModelPlasmodium FalciparumHIV-1 InteractionsHuman Primary Monocyte-derived Immune CellsMalariaHIV-1Co-infectionsDisease ProgressionTransmissionImmunological MechanismsSoluble Malarial AntigensHIV-1 InfectionReactivationImmune CellsP. Falciparum Schizont Stage ParasitesPeripheral Blood Mononuclear Cells
