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Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon
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Journal JoVE Bio-ingénierie
Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

Engineering Adherent Bacteria by Creating a Single Synthetic Curli Operon

DOI:
10.3791/4176-v

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15:28 min

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November 16, 2012

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, , , ,
1UMR CNRS 5557 Ecologie Microbienne, Université Lyon 1,Université de Lyon, 2Département Biosciences, INSA de Lyon,Université de Lyon, 3INSERM U758, Ecole Normale Supérieure de Lyon,Université de Lyon, 4Laboratoire de Génie Civil et Ingénierie Environnementale, INSA de Lyon,Université de Lyon

Chapitres

  • 00:05Titre
  • 01:55Designing the Biobrick Synthetic Curli Operon
  • 06:06Quantifying and Visualizing Adherent Bacteria on Polystyrene
  • 09:01Visualizing Adherent Bacteria on Glass by Microscopy
  • 12:22Results: Increased Adherence of Engineered Bacteria
  • 14:28Conclusion

Summary

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Traduction automatique

The design of a synthetic operon encoding both the secretory apparatus and the structural monomers of curli fibers is described. Overproduction of these amyloids and adherent polymers allows a measurable gain of adherence of the E. coli chassis1. Easy ways to visualize and quantify adherence are explained.

Tags

IngénierieAdherent BacteriaSynthetic Curli OperonE. ColiPromoterVisualizationQuantificationBacterial AdherenceSynthetic BiologyBBa K540000 BiobrickTranscriptional SignalsTranslational SignalsCurli RegulationMetal-regulated PromoterQualitative AssessmentQuantitative AssessmentBiofilm Formation

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