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A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.

DOI:
10.3791/4177-v

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12:11 min

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July 09, 2012

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, , , ,
1National Exposure Research Laboratory,Office of Research and Development, US Environmental Protection Agency, 2Shaw Environmental & Infrastructure, 3Office of Ground Water and Drinking Water,US Environmental Protection Agency

Chapitres

  • 00:05Titre
  • 01:18Tangential Flow Hollow-fiber Ultrafiltration Procedure
  • 05:08Immunomagnetic Separation Procedure
  • 08:58Staining and Examination
  • 10:20Results: Visualization of Giardia cysts and Cryptosporidium oocysts
  • 11:40Conclusion

Summary

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This protocol describes the use of a tangential flow hollow-fiber ultrafiltration sample concentration system and a heat dissociation as alternative steps for the detection of waterborne Cryptosporidium and Giardia species using EPA Method 1623.

Tags

Modified EPA Method 1623Tangential Flow Hollow-fiber UltrafiltrationHeat DissociationWaterborne CryptosporidiumGiardia Spp.ProtozoaDiarrheal Disease OutbreaksPrevalenceMonitoringUS Drinking Water SuppliesSample ConcentrationImmunomagnetic Separation ProcedureAcid Dissociation ProcedureImmunofluorescence Staining And EnumerationEnvirochek FiltersEnvirochek HV FiltersFilta-Max Filters

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