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Chapitres
- 00:05Titre
- 02:22Preparing Intact Muscle Bundles
- 05:55Measuring Contractility of the Isolated Skeletal Muscles
- 11:25Results: Ex Vivo Assessment of Contractility, Fatigability and Alternans in Murine Skeletal Muscles
- 12:19Conclusion
We describe a method to directly measure muscle force, muscle power, contractile kinetics and fatigability of isolated skeletal muscles in an in vitro system using field stimulation. Valuable information on Ca2+ handling properties and contractile machinery of the muscle can be obtained using different stimulating protocols.
Tags
Ex Vivo AssessmentContractilityFatigabilityAlternansIsolated Skeletal MusclesMuscle ForceMuscle PowerContractile KineticsRecovery After FatigueExcitation-contraction Coupling (ECC)ExcitabilityContractile MachineryCa2+ Handling AbilityGenetic ComponentsCa2+ Signaling PathwaysSkeletal Muscle PhenotypeIn Vitro Muscle Contractility AssaySingle Cell AssaysGenetic ApproachesBiochemistry AssaysMechanisms Of ECC
