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Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue
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Journal JoVE Médecine
Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

Isolation of Cardiomyocyte Nuclei from Post-mortem Tissue

DOI:
10.3791/4205-v

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10:12 min

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July 10, 2012

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,
1Strategic Research Center for Stem Cell Biology and Cell Therapy,University of Lund, 2Department of Cardiology Lund University Hospital,University of Lund

Chapitres

  • 00:05Titre
  • 02:04Isolation of the Cardiac Nuclei
  • 04:43Immunostaining for Flow Cytometry
  • 05:55Flow Cytometry
  • 07:51Results: Cardiomyocyte Nuclei Isolation by PCM-1 Positivity
  • 09:50Conclusion

Summary

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Cardiac nuclei are isolated via density sedimentation and immunolabeled with antibodies against pericentriolar material 1 (PCM-1) to identify and sort cardiomyocyte nuclei by flow cytometry.

Tags

IsolationCardiomyocyte NucleiDensity SedimentationImmunolabelingAntibodiesPericentriolar Material 1 (PCM-1)Flow Cytometry SortingHigh Throughput AnalysisFrozen Archival MaterialBiobanksCarbon-14 DatingCell-cycle AnalysisThymidine AnaloguesBrdUIdUTranscriptome AnalysisEpigenetic Analysis

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