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Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
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Médecine
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Journal JoVE Médecine
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

DOI:
10.3791/4206-v

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12:42 min

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August 28, 2012

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, ,
1Department of Preventive Medicine,University of Southern California, 2Institute for Women’s Health,University College London

Chapitres

  • 00:05Titre
  • 01:54Primary NOF Cell Line: Overexpressing hTERT
  • 04:15PolyHEMA Coating of Cell Culture Dishes
  • 05:07Generating 3D Heterotypic Spheroids
  • 06:43Re-feeding of 3D Cultures
  • 07:58Processing for Imaging and Molecular Analyses
  • 11:36Conclusion

Summary

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We describe methodologies for establishing in vitro heterotypic three-dimensional models comprising ovarian fibroblasts and normal ovarian surface or ovarian cancer epithelial cells. We discuss the use of these models to study stromal-epithelial interactions that occur during ovarian cancer development.

Tags

Heterotypic Three-dimensional In Vitro ModelingStromal-epithelial InteractionsOvarian Cancer InitiationOvarian Cancer ProgressionEpithelial Ovarian CancersGynecological MalignancySurgical TreatmentsPlatinum-based ChemotherapiesEOC Survival RatesStage I TumorsStage III/IV DiseaseMortality RatesMolecular Genetic BasisBiological BasisEarly Stage Disease DevelopmentMicroenvironment RoleRisk Factors For EOCsNormal Ovary Co-cultureOvarian Surface Epithelial CellsStromal Fibroblast Cells

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