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Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting
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Biologie
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Journal JoVE Biologie
Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

DOI:
10.3791/4214-v

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11:25 min

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October 04, 2012

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, , , ,
1School of Life Sciences,University of Warwick , 2Warwick Systems Biology,University of Warwick

Chapitres

  • 00:05Titre
  • 02:10Protoplast Generation
  • 04:23FACS of Leaf Protoplasts
  • 05:54Results: Representative Analyses of GFP Expression in Flow Sorted Arabidopsis Protoplasts
  • 10:26Conclusion

Summary

Traduction automatique

Traduction automatique

A method for producing Arabidopsis leaf protoplasts that are compatible with fluorescence activated cell sorting (FACS), allowing for studies of specific cell populations. This method is compatible with any Arabidopsis line that expresses GFP in a subset of cells.

Tags

Arabidopsis LeavesFluorescence Activated Cell SortingBiomass AccumulationCell ProliferationCell ExpansionLeaf PrimordiumLeaf OrganCell TypesLeaf Development StagesSenescenceGenomic StudyExpression DataGenetic NetworksCellular Response ZonesCell Specific Gene ExpressionLaser-capture Microdissection (LCM)GFP Expressing PlantsProtoplast GenerationINTACT SystemNuclear PrecipitationImmunoprecipitation Of Polysomes

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