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Cell specifik analyse af<em> Arabidopsis</em> Blade Med fluorescensaktiveret cellesortering
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Biologie
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Journal JoVE Biologie
Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

Cell specifik analyse af<em> Arabidopsis</em> Blade Med fluorescensaktiveret cellesortering

DOI:
10.3791/4214-v

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11:25 min

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October 04, 2012

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, , , ,
1School of Life Sciences,University of Warwick , 2Warwick Systems Biology,University of Warwick

Chapitres

  • 00:05Titre
  • 02:10Protoplast Generation
  • 04:23FACS of Leaf Protoplasts
  • 05:54Results: Representative Analyses of GFP Expression in Flow Sorted Arabidopsis Protoplasts
  • 10:26Conclusion

Summary

Traduction automatique

Traduction automatique

Fremgangsmåde til fremstilling<em> Arabidopsis</em> Blad-protoplaster, der er kompatible med fluorescensaktiveret cellesortering (FACS), hvilket muliggør undersøgelser af specifikke cellepopulationer. Denne fremgangsmåde er kompatibel med alle<em> Arabidopsis</em> Linje, der udtrykker GFP i en undergruppe af celler.

Tags

Arabidopsis LeavesFluorescence Activated Cell SortingBiomass AccumulationCell ProliferationCell ExpansionLeaf PrimordiumLeaf OrganCell TypesLeaf Development StagesSenescenceGenomic StudyExpression DataGenetic NetworksCellular Response ZonesCell Specific Gene ExpressionLaser-capture Microdissection (LCM)GFP Expressing PlantsProtoplast GenerationINTACT SystemNuclear PrecipitationImmunoprecipitation Of Polysomes

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