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Immunology and Infection

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JoVE Journal Immunology and Infection
Mass Spectrometric Analysis of Glycosphingolipid Antigens
 

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Article DOI: 10.3791/4224-v • 13:09 min • April 16th, 2013
April 16th, 2013 •
1, 2, 3,4
1Undergraduate Program, Rice University, 2Proteomics Facility, Department of Pathology, University of Texas MD Anderson Cancer Center, 3Department of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, 4University of Texas Graduate School of Biological Sciences at Houston

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résumé
Automatic Translation

A specific and sensitive method to gain insight into the expression profile of glycosphingolipid antigens in immune organs and cells is described. The method takes advantage of the ion trap mass spectrometry allowing step-wise fragmentation of glycosphingolipid molecules for structural analysis in comparison to chemically synthesized standards.

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Mass Spectrometric Analysis Glycosphingolipid Antigens GSL's Glycoconjugate Biomacromolecules Structural Information Embryonic Development Signal Transduction Immune Receptor Recognition Sugar Moieties Isomers Lipid Moieties Fatty Acyl Chain Length Unsaturation Hydroxylation Carbohydrate Portion Ceramide Portion Tri-hexosylceramides Globotriaosylceramide (Galα4Galβ4Glcβ1Cer) Isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer) Molecular Masses Sugar Linkages Biological Functions Modification Of Ceramide Part Alpha-galactosylceramide Cytokine Secretion Profiles Invariant NKT Cells Cancer Models Autoimmune Diseases Structural Analysis Of Isomers Immune Organs And Cells
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