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Manual Drainage of the Zebrafish Embryonic Brain Ventricles
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Journal JoVE Neurosciences
Manual Drainage of the Zebrafish Embryonic Brain Ventricles

Manual Drainage of the Zebrafish Embryonic Brain Ventricles

DOI:
10.3791/4243-v

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06:17 min

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December 16, 2012

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,
1Department of Biology, Whitehead Institute of Biomedical Research,Massachusetts Institute of Technology

Chapitres

  • 00:05Titre
  • 01:21Preparing Microinjection Needles, Oil Injector Apparatus, and Embryos
  • 02:28Draining and Collecting the Embryonic Cerebrospinal Fluid (eCSF)
  • 04:24Representative eCSF Drainage Results
  • 05:55Conclusion

Summary

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Traduction automatique

We present a method to collect cerebrospinal fluid (CSF) and to create a system which lacks CSF within the embryonic zebrafish brain ventricular system. This allows for further examination of CSF composition and its requirement during embryonic brain development.

Tags

Manual DrainageZebrafish Embryonic Brain VentriclesCerebrospinal FluidProtein-rich FluidVertebrate Embryonic DevelopmentChoroid PlexusHighly Vascularized Secretory RegionsNeuroepitheliumEmbryonic CSFECSFAdult CSFSecreted FactorsNeurogenesisGene ExpressionCell ProliferationCell SurvivalProteomic Analyses

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