Journal
/
Immunologie et infection
/
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry
Journal JoVE
Immunologie et infection
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

Chemoselective Modification of Viral Surfaces via Bioorthogonal Click Chemistry

DOI:
10.3791/4246-v

•

12:31 min

•

August 19, 2012

•

, , , ,
1Department of Chemistry,Stony Brook University

Chapitres

  • 00:00Titre
  • 02:04Production of Aha-Labeled Adenovirus
  • 04:28Production of Azido-Sugar-Labeled Adenovirus
  • 05:46Purification of Azide-Labeled Adenoviral Particles
  • 07:34Ligation via Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)
  • 08:32Ligation via Copper(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC)
  • 09:32SDS-PAGE Analysis of Clicked Adenovirus
  • 10:34Conclusion

Summary

Traduction automatique

Traduction automatique

Adenovirus particles are engineered to contain either the unnatural amino acid analogue azidohomoalanine or the azido sugar O-GlcNAz. The azide group of each is chemoselectively ligated via "click" chemistry reactions as a means of viral surface modification.

Tags

Chemoselective ModificationViral SurfacesBioorthogonal Click ChemistryGene TherapyOncolytic ApplicationsVaccine DevelopmentGenetics-based ApproachesChemoselective LigationTargeting MoleculesDyesAzide TagsMetabolic LabelingEffector LigandsAdenovirus ParticlesAzidohomoalanineO-linked N-azidoacetylglucosamine

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/4246?article_type=v

Using a Pan-Viral Microarray Assay (Virochip) to Screen Clinical Samples for Viral Pathogens
check_url/fr/4246?article_type=v

Isolation of Fidelity Variants of RNA Viruses and Characterization of Virus Mutation Frequency
check_url/fr/4246?article_type=v

Preparation of Viral DNA from Nucleocapsids
check_url/fr/4246?article_type=v

Production and Titering of Recombinant Adeno-associated Viral Vectors
check_url/fr/4246?article_type=v

piggyBac Transposon System Modification of Primary Human T Cells
check_url/fr/4246?article_type=v

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
check_url/fr/4246?article_type=v

Using Click Chemistry to Measure the Effect of Viral Infection on Host-Cell RNA Synthesis
check_url/fr/4246?article_type=v

The Development of Lyophilized Loop-mediated Isothermal Amplification Reagents for the Detection of Coxiella burnetii
check_url/fr/4246?article_type=v

Measuring Influenza Neuraminidase Inhibition Antibody Titers by Enzyme-linked Lectin Assay
check_url/fr/4246?article_type=v

Swab Sampling Method for the Detection of Human Norovirus on Surfaces

Read Article