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Ex vivo Culturing of Whole, Developing Drosophila Brains

Journal JoVE
Neurosciences

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Journal JoVE Neurosciences

Ex vivo Culturing of Whole, Developing Drosophila Brains

Ex vivo Culturing of Whole, Developing Drosophila Brains

DOI:

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08:39 min

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July 27, 2012

•

Ranjini Prithviraj², Svetlana Trunova², Edward Giniger²

¹National Institute of Neurological Disorders and Stroke, ²National Human Genome Research Institute,National Institutes of Health, Bethesda, MD

Chapitres

00:05Titre
01:22Culturing Larval Brains
02:23Culturing Pupal Brains
03:30Fixing, Staining, and Mounting Larval and Pupal Brains
04:38Representative Brain Culturing Results
08:03Conclusion

Summary

Traduction automatique

This article describes a method by which one can mimic in vivo development of the Drosophila mushroom body in an ex vivo culture system.

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