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Patch Clamp Recordings i Indre ørehår celler isolert fra sebrafisk
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Neurosciences
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Journal JoVE Neurosciences
Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish

Patch Clamp Recordings i Indre ørehår celler isolert fra sebrafisk

DOI:
10.3791/4281-v

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12:43 min

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October 17, 2012

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, , , , , , ,
1Natural Science Division,Pepperdine University

Chapitres

  • 00:05Titre
  • 01:52Pre-experimental Preparations
  • 02:47Isolation of Auditory and Vestibular Labyrinth
  • 05:28Hair Cell Isolation
  • 07:18Patch Clamping Zebrafish Hair Cells
  • 10:12Results: Isolation of and Electrophysiological Recordings from Zebrafish Hair Cells
  • 12:04Conclusion

Summary

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Formålet med denne videoen er å demonstrere prosedyrer for å innhente sunne, intakte hårceller fra det indre øret organer av voksen sebrafisk og deretter bruke dem for patch clamp studier med sikte på å karakterisere biofysiske egenskaper deres spenningsstyrte kanaler.

Tags

Patch Clamp RecordingsInner Ear Hair CellsZebrafishVoltage-gated ChannelsSensory Hair CellsIsolation MethodHair Cell SizeHair Cell MorphologyDiversityPatch Clamp RecordingsZebrafish Model SystemMutantsHearing And BalanceTransgenic LinesGenetic AnalysisElectrophysiological MethodsSensory Modalities

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