Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Research Service,Veterans Affairs Greater Los Angeles Healthcare System, 2Department of Urology, The David Geffen School of Medicine,University of California, Los Angeles , 3GeneFluidics, 4Division of Infectious Diseases,Veterans Affairs Greater Los Angeles Healthcare System, 5Department of Microbiology, Immunology & Molecular Genetics,University of California, Los Angeles
Chapitres
- 00:05Titre
- 01:39Functionalization of Electrochemical Sensors
- 03:02Sample Preparation
- 03:55Electrochemical Sensor Assay
- 05:14Results: Bacterial Detection & Identification Using Electrochemical Sensors
- 08:46Conclusion
We describe an electrochemical sensor assay method for rapid bacterial detection and identification. The assay involves a sensor array functionalized with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. Sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase-linked DNA oligonucleotide detector probe produces a measurable amperometric current.
Tags
Bacterial DetectionIdentificationElectrochemical SensorsBlood Glucose MeasurementAnalyte DetectionSignal TransductionRedox EnzymeAmperometric ElectrodeSensor SpecificityGlucose SensorDNA OligonucleotidesSandwich HybridizationRibosomal RNA (rRNA)Capture ProbeDetector ProbeHorseradish Peroxidase (HRP)Substrate OxidationCurrent Flow
