Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Anesthesiology,David Geffen School of Medicine at UCLA, 2Department of Medicine,David Geffen School of Medicine at UCLA, 3Department of Physiology,David Geffen School of Medicine at UCLA, 4Department of Internal Medicine,Nora Eccles Harrison Cardiovascular Research and Training Institute, University of Utah
Chapitres
- 00:05Titre
- 01:06Heart Homogenization and Nuclear Isolation
- 02:22Nucleoplasm and Detergent-extracted Chromatin Fractionation
- 03:22Acid-extraction Fractionation – DNA-bound Protein Enrichment
- 04:51Representative Chromatin Proteome Quantitation
- 07:49Conclusion
Fremskridt inden massespektrometri har tilladt high throughput analyse af proteinekspression og ændringen i en lang række væv. Kombineret med subcellulær fraktionering og sygdomsmodeller, kvantitativ massespektrometri og bioinformatik kan afsløre nye egenskaber i biologiske systemer. Fremgangsmåden beskrevet heri analyseres chromatin-associerede proteiner i tilfælde af hjertesygdomme og let anvendes til andre<em> In vivo</em> Modeller af sygdomme hos mennesker.
Tags
Quantitative AnalysisChromatin ProteomesDiseaseNucleusGene RegulationAdult Mammalian Cardiomyocyte NucleiBinucleated CellsHeterochromatic StateDNA PackagingTranscriptional RegulationDevelopmentDiseaseNuclear ProteinsTranscriptional ProgramsHeart DiseaseMortalityNuclear ProteomeMass SpectrometryAnnotationRelative QuantificationProtein AbundanceProteomic StudiesCardiac Nuclear ProteomeNuclear Sub Compartments
