Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

Anastacia M. Garcia; Mary L. Ladage; Pamela A. Padilla

doi:10.3791/4319

Journal

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Biologie

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Brug af Time Lapse Microscopy at Visualiser Anoxia-induceret Suspended Animation i C. elegans Embryoner

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Biologie

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Journal JoVE Biologie

Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

Brug af Time Lapse Microscopy at Visualiser Anoxia-induceret Suspended Animation i C. elegans Embryoner

DOI:

10.3791/4319-v

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08:49 min

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December 03, 2012

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Anastacia M. Garcia, Mary L. Ladage, Pamela A. Padilla

¹Department of Biological Sciences,University of North Texas

Chapitres

00:05Titre
02:02Sample Preparation
03:15Slide Preparation and Anoxia Chamber Setup
05:52Microscopy in Anoxia
07:36Representative Embryo Imaging Results
08:10Conclusion

Summary

Traduction automatique

Beskrevet her er en In vivo Teknik til billede subcellulære strukturer i dyr udsat for anoxi ved hjælp af en gasstrøm gennem microincubation kammer i forbindelse med en roterende skive konfokalt mikroskop. Denne metode er enkel og fleksibel nok til at passe til en række af eksperimentelle parametre og modelsystemer.

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Brug af Time Lapse Microscopy at Visualiser Anoxia-induceret Suspended Animation i<em> C. elegans</em> Embryoner

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