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Bruk av Time Lapse Mikroskopi å visualisere anoxia-indusert suspendert animasjon i<em> C. elegans</em> Embryoer
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Journal JoVE Biologie
Use of Time Lapse Microscopy to Visualize Anoxia-induced Suspended Animation in C. elegans Embryos

Bruk av Time Lapse Mikroskopi å visualisere anoxia-indusert suspendert animasjon i<em> C. elegans</em> Embryoer

DOI:
10.3791/4319-v

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08:49 min

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December 03, 2012

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, ,
1Department of Biological Sciences,University of North Texas

Chapitres

  • 00:05Titre
  • 02:02Sample Preparation
  • 03:15Slide Preparation and Anoxia Chamber Setup
  • 05:52Microscopy in Anoxia
  • 07:36Representative Embryo Imaging Results
  • 08:10Conclusion

Summary

Traduction automatique

Traduction automatique

Beskrevet her er en<em> In vivo</em> Teknikk til bildet sub-cellulære strukturer i dyr eksponert for anoksi hjelp en gasstrøm gjennom microincubation kammer i forbindelse med en roterende plate konfokal mikroskop. Denne metoden er enkel og fleksibel nok til å passe en rekke eksperimentelle parametre og modellere systemer.

Tags

Time Lapse MicroscopyAnoxia-induced Suspended AnimationC. Elegans EmbryosStress ResistanceGenetic MutantsTransgenic StrainsFusion ProteinsFluorescently Labeled Reporter ProteinsMitosisIn Vivo ImagingSub-cellular StructuresAnoxic StressGas Flow SetupHigh-powered MicroscopeMicroincubation ChamberNitrogen Gas Flow ThroughSpinning Disc Confocal MicroscopeControlled EnvironmentGFP-tagged Gamma TubulinHistoneCell Division ArrestOxygen Deprivation

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