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Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
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Journal JoVE Biologie
Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format

DOI:
10.3791/4335-v

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07:27 min

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March 05, 2013

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, ,
1Max Planck Institute for Molecular Biomedicine, 2Medical Faculty,University of Münster

Chapitres

  • 00:05Titre
  • 01:25Embryoid Body Formation
  • 03:04Neuroectoderm Induction and Neuron Formation
  • 04:25Representative Neuron Generation Results
  • 07:06Conclusion

Summary

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Traduction automatique

Protocols for neuronal differentiation of pluripotent human stem cells (hPSCs) are often time-consuming and require substantial cell culture skills. Here, we have adapted a small molecule-based differentiation procedure to a multititre plate format, allowing simple, rapid, and efficient generation of human neurons in a controlled manner.

Tags

NeuronsHuman Pluripotent Stem CellsMultititre Plate FormatProtocolsGenerationNeuroectodermDifferentiationSignaling PathwaysTGFβ/SMAD2BMP/SMAD1FGF/ERKFunctional NeuronsReproducibilityMid-throughput ApplicationsFloating SpheresAdherent ConditionsBipolar Sensory NeuronsEfficient ProcedureChemically Defined MediumSmall MoleculesFunctional InvestigationCell-based Screening Approaches

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