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Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
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Journal JoVE Biologie
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR

DOI:
10.3791/4356-v

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14:07 min

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November 23, 2012

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,
1The Philips Institute of Oral and Craniofacial Molecular Biology,Virginia Commonwealth University

Chapitres

  • 00:05Titre
  • 01:24High-throughput PCR Amplification and Purification
  • 06:24Preparation of Competent Cells, Cell Transformation and Antibiotic Selection
  • 08:30Mutant Confirmation and Storage
  • 10:34Results: Creation of Recombinant PCR Constructs and Correct Mutants
  • 13:48Conclusion

Summary

Traduction automatique

Traduction automatique

An efficient genome-wide single gene mutation method has been established using Streptococcus sanguinis as a model organism. This method has achieved via high throughput recombinant PCRs and transformations.

Tags

Genome-wide Gene DeletionsStreptococcus SanguinisHigh Throughput PCRTransposon MutagenesisSingle-gene DeletionBacteriaGene KnockoutMutant LibraryPartial Gene InactivationHigh-throughput TechniqueModel OrganismGene Deletion ConstructKanamycin Resistance ProteinPrimersPCR Amplification

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