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Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
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Médecine
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Journal JoVE Médecine
Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

DOI:
10.3791/4372-v

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14:52 min

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November 24, 2012

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, ,
1Department of Stem Cells and Developmental Biology, Cell Science Research Center,Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran, 2Department of Endocrinology & Female Infertility, Reproductive Biomedicine Center,Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran

Chapitres

  • 00:00Titre
  • 01:18Introduction
  • 03:16Preparation of Enzyme Solution
  • 04:06Human Dental Pulp Isolation
  • 04:48Isolation of Dental Pulp Stem Cells by Enzymatic Dissociation of Dental Pulp Tissue
  • 06:28Outgrowth of Stem Cells from Tissue Explants
  • 07:27Immunophenotyping
  • 08:26Inducing Odontoblast Differentiation & Alizarin Red Staining Assay
  • 12:21Dental Pulp Stem Cells Culture and Differentiation Results
  • 14:14Conclusion

Summary

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The method described isolation and characterization of human Dental Pulp Stem Cells (hDPSCs) by using either enzymatic dissociation of pulp (DPSC-ED) or direct outgrowth of stem cells from pulp tissue explants (DPSC-OG). Then followed by in vitro comparative differentiation of both types of hDPSCs into odontoblasts.

Tags

IsolationCharacterizationComparative DifferentiationHuman Dental Pulp Stem CellsPermanent TeethTwo Different MethodsWisdom TeethRoutine Orthodontic TreatmentsHDPSCsProliferation PotentialMulti-lineage Differentiation CapacityAutologous TransplantationRegenerative MedicineMesenchymal Stem CellsMSC FeaturesImmunomodulatory EffectAllograft TransplantationIsolation ConditionsSurface MarkersDifferentiation CapacityImpacted Third Molar TeethDental Diamond Disk

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