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1Department of Biochemistry, Microbiology and Immunology,Neural Regeneration Laboratory and Ottawa Institute of Systems Biology, 2Department of Cellular and Molecular Medicine,University of Ottawa , 3CIHR Program in Neurodegenerative Lipidomics,University of Ottawa , 4Carleton Immersive Media Studio,Azrieli School of Architecture and Urbanism
Chapitres
- 00:00Titre
- 02:48Introduction
- 03:26Preparing Reagents
- 03:46Obtaining the Vaginal Smear
- 05:06Cytological Staining using Crystal Violet
- 05:46Vaginal Cytology
- 07:13Conclusion
Her beskriver vi hvordan du kan identifisere stadium av murine reproduktive (proestrus, estrus, metestrus eller diestrus) med enkle, ikke-invasiv innsamling og cytologisk vurdering av vaginal smear prøver. Vi videre beskrive hvordan vaginal cytologi reflekterer sirkulerende hormonelle nivåer underliggende overgang gjennom murine reproduktive syklus.
Tags
Vaginal LavageCrystal Violet StainingVaginal Cytological EvaluationMouse Estrous Cycle StagingReproductive StatusMouse Models Of DiseaseHormonal RegulationPathological ChallengeProestrusEstrusMetestrusDiestrusOvarian Steroids17-beta-estradiolProgesteroneLuteinizing HormoneFollicle Stimulating HormoneProlactinCell TypologyNucleated Epithelial CellsCornified Squamous Epithelial CellsLeukocytesVaginal SmearsInvasivenessNon-invasive Protocol
