Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:43Clipping, Loading, and Calibration of Microgavage Needles
- 03:50Anesthetizing, Mounting, and Gavage of Zebrafish Larvae
- 07:02Dextran Assay to Test Gavage Safety and Intestinal Barrier Integrity
- 08:41Evaluation of Dextran Assay for Epithelial Barrier Integrity
- 10:10Conclusion
We present a novel method for microgavage of larval zebrafish utilizing standard embryo microinjection and stereomicroscopy equipment. We demonstrate that microgavage is a safe and efficient technique useful for delivering controlled amounts of diverse materials specifically into the larval zebrafish intestinal lumen.
Tags
Zebrafish LarvaeIntestinal DevelopmentPhysiologyDiseaseHost-microbe InteractionsExperimental ApproachesIn Vivo IntroductionSelected MaterialsLumen Of The IntestineImmersion MethodInjectionAbdominal WallMaterial DeliveryUnintended ToxicityMaterial AmountsIngestion VariabilityDirect InjectionMicrogavage MethodSafe DeliveryConsistent Delivery TimingEmbryo MicroinjectionStereomicroscopy Equipment
