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Fremstilling af cellelinier til Betinget Knockdown af genekspression og måling af Knockdown Virkninger på E4orf4-induceret celledød
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Journal JoVE Biologie
Preparation of Cell-lines for Conditional Knockdown of Gene Expression and Measurement of the Knockdown Effects on E4orf4-Induced Cell Death

Fremstilling af cellelinier til Betinget Knockdown af genekspression og måling af Knockdown Virkninger på E4orf4-induceret celledød

DOI:
10.3791/4442-v

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13:54 min

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October 21, 2012

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, ,
1Department of Molecular Microbiology, Faculty of Medicine,Technion – Israel Institute of Technology

Chapitres

  • 00:05Titre
  • 01:50Generation of Inducible Cell Lines
  • 06:19Induction of Knockdown and Transfection
  • 08:08DAPI Assay in Transfected Cells
  • 10:59Results: Detection of E4orf4-induced Cell Death in Inducible Cell Lines
  • 13:06Conclusion

Summary

Traduction automatique

Traduction automatique

Bidrag ACF chromatin remodeling faktor til E4orf4-induceret celledød blev målt. Protokollen omfatter selektion af cellekloner, hvor doxycyclin behandling inducerer betinget knockdown af ACF-underenheder Acf1 og SNF2h, og brugen af ​​DAPI assay til måling af E4orf4-induceret celledød i de inducerbare cellelinjer.

Tags

Conditional KnockdownGene ExpressionCell LinesACF Chromatin Remodeling FactorDoxycycline-inducible PromoterNeomycin Resistance GeneShRNA ConstructsAcf1SNF2hE4orf4 ProteinCell Death

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