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Sıralı Nöromusküler Kavşağı'nda Tek Schwann Hücreleri belirginleştiren Photo-beyazlatma
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1Lehrstuhl für Biomolekulare Sensoren,Technische Universität München, 2Center for Integrated Protein Science (Munich) at the Institute of Neuroscience,Technische Universität München, 3TUM Institute for Advanced Study and German Center for Neurodegenerative Diseases,Technische Universität München, 4Munich Cluster for Systems Neurology (SyNergy),Technische Universität München
Chapitres
- 00:05Titre
- 01:36Preparing the Triangularis Sterni Explant
- 04:34Photobleaching and Time-lapse Microscopy
- 07:52Fixing and Dissecting the Explant for Immunohistochemistry
- 09:45Triangularis Sterni Visualization with GFP in Schwann Cells
- 10:42Conclusion
Gibi nöromüsküler kavşaklar (NMJs) terminal Schwann hücreleri (SC'ler) gibi sık donatılı dokularda, tek tek hücrelerin Görselleştirme zordur. "Ardışık fotoğraf beyazlatma" bleaching sıralı time-lapse görüntüleme ile kombine edilebilir triangularis sternumun kas eksplant, elverişli bir sinir-kas preparatı,, örneğin tek bir terminal SC'ler, tasvir verir ve<em> Post-hoc</em> Immunostainings.
Tags
Sequential Photo-bleachingSchwann CellsNeuromuscular JunctionMotor NeuronsTransgenic MiceSynaptic StructureSynaptic FunctionMyelinating Axonal SCsNodes Of RanvierSaltatory Action Potential PropagationGlial CellsNeurotransmissionDebris DigestionAxon RegenerationNon-myelinating Terminal SCsLight MicroscopyDye FillingTime-lapse RecordingsMulti-spectral Labeling
