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Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes

DOI:

10.3791/50044-v

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07:25 min

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March 29, 2013

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Nancy Jo Pokrywka

¹Department of Biology,Vassar College

Chapitres

00:05Titre
01:28Preparing Flies for Dissection
02:15Preparation of Oocytes for Imaging Using an Upright Compound Microscope
04:33Live Imaging
06:18Results: Live Imaging of Autofluorescent and GFP-tagged Structures
07:00Conclusion

Summary

Traduction automatique

A protocol for live imaging of GFP-tagged proteins or autofluorescent structures in individual Drosophila oocytes is described.

Tags

Keywords: Drosophila Oocytes Live Imaging GFP-labeled Proteins Cytoskeleton Ooplasmic Streaming Confocal Microscopy

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