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Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols
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Immunologie et infection
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Journal JoVE Immunologie et infection
Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

DOI:
10.3791/50075-v

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11:31 min

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January 02, 2013

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, , , ,
1Centre for Blood Research,University of British Columbia , 2Department of Pathology and Laboratory Medicine,University of British Columbia , 3Canadian Blood Services,University of British Columbia , 4Department of Chemistry, Life Sciences Centre,University of British Columbia

Chapitres

  • 00:05Titre
  • 01:28Succinimidyl Succinate-Hyperbranched Polyglycerol Modification (SS-HPG)
  • 03:30Whole Blood Collection and Separation of Red Blood Cells (RBC)
  • 04:26HPG Grafting to RBC
  • 05:28Characterization of HPG Modified RBC: Complement Mediated Lysis
  • 07:16Characterization of HPG Modified RBC: The Camouflage of Major and Minor Ag using MTS Cards
  • 08:08Characterization of HPG Modified RBC: Aqueous Two Phase Partition Measurements
  • 09:14Characterization of HPG Modified RBC: Flow Cytometry Measurements – Protection of Rhesus-D (RhD) Antigens
  • 10:12Results: Representative Evaluation of the Camouflage of Surface Antigens and Proteins using Flow Cytometry
  • 10:58Conclusion

Summary

Traduction automatique

Traduction automatique

The cell membrane modification of red blood cells (RBCs) with hyperbranched polyglycerol (HPG) is presented. Modified RBCs were characterized by aqueous two phase partitioning, osmotic fragility and complement mediated lysis. The camouflage of surface proteins and antigens was evaluated using the flow cytometry and Micro Typing System (MTS) blood phenotyping cards.

Tags

Keywords: Antigen ProtectionRed Blood CellsMembrane GraftingHyperbranched PolyglycerolAlloantibodiesUniversal Red Blood DonorTransfusion Safety

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