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Immunology and Infection

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JoVE Journal Immunology and Infection
Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro
 

Establishing a Liquid-covered Culture of Polarized Human Airway Epithelial Calu-3 Cells to Study Host Cell Response to Respiratory Pathogens In vitro

Article DOI: 10.3791/50157-v • 12:09 min • February 7th, 2013
February 7th, 2013 •
1, 1
1National Center for Immunization and Respiratory Diseases, Division of Viral Diseases, Gastroenteritis and Respiratory Viruses Laboratory Branch, Centers for Disease Control and Prevention (CDC)

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The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

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Liquid-covered Culture Polarized Human Airway Epithelial Cells Calu-3 Cells Host Cell Response Respiratory Pathogens In Vitro Model Respiratory Syncytial Virus (RSV) Influenza Virus Rhinovirus Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV) Air-liquid Interface Culture Normal Human Bronchial Epithelial Cells (NHBE) Lung Tissue Samples Culturing Epithelial Cells Pharmacological Compounds Bacterial Pathogens Viral Pathogens
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