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Immunology and Infection
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JoVE Journal Immunology and Infection
Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs
 

Ex Vivo Red Blood Cell Hemolysis Assay for the Evaluation of pH-responsive Endosomolytic Agents for Cytosolic Delivery of Biomacromolecular Drugs

Article DOI: 10.3791/50166-v • 11:13 min • March 9th, 2013
March 9th, 2013 •
*1,2, *1,2, *1,2, 2,3, 1, 1,2, 4, 1,2,5,6, 1,2
1Department of Biomedical Engineering, Vanderbilt University, 2Vanderbilt Institute for Nanoscale Science & Engineering, Vanderbilt University, 3Interdisciplinary Materials Science Program, Vanderbilt University, 4Monroe Carell Jr. Children's Hospital, Vanderbilt University Medical Center, 5Department of Chemical & Biomolecular Engineering, Vanderbilt University, 6Department of Cancer Biology, Vanderbilt University
* These authors contributed equally

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résumé
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A hemolysis assay can be used as a rapid, high-throughput screen of drug delivery systems' cytocompatibility and endosomolytic activity for intracellular cargo delivery. The assay measures the disruption of erythrocyte membranes as a function of environmental pH.

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Ex Vivo Red Blood Cell Hemolysis Assay PH-responsive Endosomolytic Agents Cytosolic Delivery Biomacromolecular Drugs Phospholipid Bilayers Intracellular Targets Synthetic Drug Carriers Endosomal Membrane Disruption Cargo Delivery Hemolysis Assay Cytocompatibility Endosomolytic Activity Drug Delivery Systems Red Blood Cells Buffers At Defined PHs Extracellular Environment Early Endosomal Environment Late Endo-lysosomal Environment Centrifugation Step Hemoglobin Release Measurement Erythrocyte Membrane Surrogate Lipid Bilayer Membrane Physiologic PH Endo-lysosomal PH Range
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