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Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells
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Journal JoVE Biologie
Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

Isolation and Differentiation of Stromal Vascular Cells to Beige/Brite Cells

DOI:
10.3791/50191-v

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07:22 min

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March 28, 2013

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, ,
1UCSF Diabetes Center and Department of Cell and Tissue Biology,University of California, San Francisco , 2Department of Biology,University of Copenhagen, Denmark, 3National Institute of Nutrition and Seafood Research, Bergen, Norway

Chapitres

  • 00:05Titre
  • 01:16Adipose Tissue Isolation
  • 02:13Tissue Cutting and Digestion
  • 03:09Preparing Cell Suspension
  • 03:58Plating Cells
  • 04:46Differentiate Cells
  • 05:47Results: Browning of Primary Adipocytes
  • 07:02Conclusion

Summary

Traduction automatique

Traduction automatique

Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. Presented here is a reliable cellular model system to study the molecular regulation of "browning" of white fat.

Tags

Beige AdipocytesBrite AdipocytesStromal Vascular FractionPreadipocytesUCP1PPAR-gammaObesity TreatmentCell DifferentiationPrimary Adipocytes

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