Journal
/
Chimie
/
Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
Journal JoVE
Chimie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Chimie
Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

DOI:
10.3791/50225-v

•

05:52 min

•

February 04, 2013

•

,
1Department of Chemistry,University of Virginia

Chapitres

  • 00:05Titre
  • 01:33Benedict’s Assay
  • 02:50Bial’s Orcinol Assay
  • 03:45Dische’s Diphenylamine Assay
  • 04:26Representative Assay Results
  • 05:36Conclusion

Summary

Traduction automatique

Traduction automatique

A suite of colorimetric assays is described for rapidly distinguishing protein, RNA, DNA, and reducing sugars in potentially heterogeneous biomolecular samples.

Tags

Colorimetric AssayRNADNABiomolecular SampleSpectrophotometricFluorometricNucleic AcidProteinBenedict’s ReagentBial’s ReagentDische’s ReagentPentoseCarbohydrateReducing Sugar

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Rapid High Throughput Amylose Determination in Freeze Dried Potato Tuber Samples

Formation of Ordered Biomolecular Structures by the Self-assembly of Short Peptides

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures

Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

Deacetylation Assays to Unravel the Interplay between Sirtuins (SIRT2) and Specific Protein-substrates

A Method to Manipulate Surface Tension of a Liquid Metal via Surface Oxidation and Reduction

Coulomb Explosion Imaging as a Tool to Distinguish Between Stereoisomers

DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

Rapid Nanoprobe Signal Enhancement by In Situ Gold Nanoparticle Synthesis

NMR-Based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Read Article