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Giant liposomfremstilling for billedbehandling og Patch-Clamp Elektrofysiologi
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Journal JoVE Biologie
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology

Giant liposomfremstilling for billedbehandling og Patch-Clamp Elektrofysiologi

DOI:
10.3791/50227-v

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09:03 min

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June 21, 2013

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,
1Department of Physiology and Biophysics,University of Washington

Chapitres

  • 00:05Titre
  • 01:19Deposition of Lipids from Organic Solvents: Classical Protocol
  • 03:13Electroformation of Giant Liposomes
  • 05:19Deposition of Lipids by Controlled Dehydration of Small Liposomes
  • 07:10Results: Electroformation of Giant Liposomes from Small Liposome Dispersions is Compatible with Reconstituted Ion Channel Function
  • 08:32Conclusion

Summary

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Færdigtilberedning funktionelle membranproteiner i kæmpeliposomer af definerede sammensætning er en kraftfuld metode, når det kombineres med patch-clamp elektrofysiologi. Dog kan konventionelle kæmpe liposomproduktion være uforeneligt med protein stabilitet. Vi beskriver protokoller til at lave kæmpeliposomer fra rene lipider eller små liposomer indeholdende ionkanaler.

Tags

Keywords: Giant LiposomesIon ChannelsElectrophysiologyPatch-clampElectroformationLipid MembranesReconstitutionSmall LiposomesDehydrationVapor EquilibriumSaturated Salt Solutions

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