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Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
 

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Article DOI: 10.3791/50238-v • 15:19 min • January 12th, 2013
January 12th, 2013 •
*1, *1, 1, 1, 1, 1, 1, 2, 3, 4, 1, 1
1Umeå Centre for Molecular Medicine, Umeå University, 2Cell Transplant Center, Diabetes Research Institute, University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation, Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science, Umeå University
* These authors contributed equally

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We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.

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Near Infrared Optical Projection Tomography β-cell Mass Distribution Diabetes Research Imaging Near Infrared Spectrum Pancreatic Tissue Computational Tools Specimen's Center Of Mass Axis Of Rotation Geometric Distortions Tomographic Reconstruction Intensity Equalization Signal To Noise Ratios Sample Holder Intact Pancreata Islet Transplantation Individual Islets Of Langerhans
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