Journal
/
Biologie
/
Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain

DOI:
10.3791/50239-v

•

11:00 min

•

May 19, 2013

•

, , , ,
1Molecular, Cellular and Developmental Biology,University of Michigan

Chapitres

  • 00:05Titre
  • 01:44Fly Husbandry and Dissection
  • 04:04Tissue Dissociation and DNA Staining
  • 05:16Flow Cytometry
  • 07:52Results: Live Cell Cycle Analysis
  • 10:41Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol for cell cycle analysis of live Drosophila tissues using the Attune Acoustic Focusing Cytometer is described. This protocol simultaneously provides information about relative cell size, cell number, DNA content and cell type via lineage tracing or tissue specific expression of fluorescent proteins in vivo.

Tags

Cell Cycle AnalysisDrosophilaFlow CytometryAttune Acoustic Focusing CytometerVybrant DyeCycle VioletDNA StainLive-cell AnalysisCell-type Specific AnalysisCell SizeCell Number

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations

Analysis of Cell Cycle Position in Mammalian Cells

Assessment of GFP Expression and Viability Using the Tali Image-Based Cytometer

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

Cytological Analysis of Spermatogenesis: Live and Fixed Preparations of Drosophila Testes

Reduced-gravity Environment Hardware Demonstrations of a Prototype Miniaturized Flow Cytometer and Companion Microfluidic Mixing Technology

LDL Cholesterol Uptake Assay Using Live Cell Imaging Analysis with Cell Health Monitoring

Read Article