Journal
/
/
Novas ferramentas para expandir as células T reguladoras de indivíduos HIV-1-infected
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
DOI:

09:27 min

May 30, 2013

, ,

Chapitres

  • 00:05Titre
  • 01:44Regulatory T cell isolation from HIV-1 Positive Blood
  • 03:40Cell Culture
  • 04:27Phenotyping
  • 05:14Suppression Assay
  • 07:18Results: Representative Treg Cells Expanded from HIV-1-infected Individuals
  • 08:33Conclusion

Summary

Traduction automatique

CD4+ Regulatory T cells are potent immune-modulators and serve important functions in immune homeostasis. The paucity of these cells in peripheral blood makes functional studies challenging, specifically in the context of HIV-1-infection. We here describe a method to isolate and expand functional CD4+ Tregs from peripheral blood from HIV-1-infected individuals.

Vidéos Connexes

Read Article