Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

Journal

Biologie

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Biologie

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Journal JoVE Biologie

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

DOI:

10.3791/50248-v

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08:36 min

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April 12, 2013

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Hong Li, Trevor Williams²

¹Department of Craniofacial Biology,University of Colorado Denver Anschutz Medical Campus, ²Department of Cell and Developmental Biology,University of Colorado Denver Anschutz Medical Campus

Chapitres

00:05Titre
01:34Dissecting CD-1 E10.5 Embryos
03:18Dispase II Treatment
04:13Separating the Facial Ectoderm and Mesenchyme
06:37Tissue Purity after Separation
07:26Conclusion

Summary

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A protocol for separation of embryo facial ectoderm and mesenchyme is described. We use Dispase II to treat whole embryos first, dissect whole facial prominences out, and then separate the facial ectoderm and mesenchyme.

Separation of Mouse Embryonic Facial Ectoderm and Mesenchyme

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Chapitres

Summary

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