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Chapitres
- 00:05Titre
- 00:59Cross-linking Chromatin
- 02:27Sonication
- 04:14Pre-clearing Chromatin
- 05:15Chromatin Immunoprecipitation
- 07:10Reversing Cross-link and Purifying DNA
- 08:12Results: Generation of a High Quality ChIP Template
- 09:28Conclusion
The combination of chromatin immunoprecipitation and ultra-high-throughput sequencing (ChIP-seq) can identify and map protein-DNA interactions in a given tissue or cell line. Outlined is how to generate a high quality ChIP template for subsequent sequencing, using experience with the transcription factor TCF7L2 as an example.
