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Cell Co-culture Patterning Using Aqueous Two-phase Systems
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Cell Co-culture Patterning Using Aqueous Two-phase Systems
DOI:

10:11 min

March 26, 2013

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Chapitres

  • 00:05Titre
  • 01:32Phase System Characterization: Determining Thresholds for Phase Separation
  • 03:22Configuration 1: Exclusion Patterning of HeLa Cells
  • 05:24Configuration 2: Island Patterning
  • 06:41Configuration 3: Exclusion / Island Patterned Co-Cultures
  • 08:08Results: Cell Patterning with the Aqueous Two-phase System
  • 09:47Conclusion

Summary

Traduction automatique

Aqueous two-phase systems were used to simultaneously pattern multiple populations of cells. This fast and easy method for cell patterning takes advantage of the phase separation of aqueous solutions of dextran and polyethylene glycol and the interfacial tension that exists between the two polymer solutions.

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