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Cellepopulasjonen Analyser Under Skin Karsinogenese
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Cell Population Analyses During Skin Carcinogenesis
DOI:

06:53 min

August 21, 2013

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Chapitres

  • 00:05Titre
  • 01:24Generating K14-creER+ R26-SmoM2YFP+ Mice and Inducing SmoM2 Expression in Skin Keratinocytes
  • 02:35Skin Specimen Preparation and Epidermis Isolation
  • 03:11Single Cell Generation
  • 03:54Cell Labeling and Flow Cytometric Analysis
  • 05:08Results: Induction of SmoM2 Expression and Skin Keratinocytes
  • 06:31Conclusion

Summary

Traduction automatique

Kreftutvikling er en prosess som involverer samspillet mellom kreftceller og kreft mikromiljøet. Å dissekere de molekylære hendelser, må man isolere ulike celle populasjoner på forskjellige stadier i kreftutvikling. Bruke en mus modell for basal cell carcinoma, beskriver vi en protokoll for celle analyser under kreftutvikling.

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