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Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord
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Journal JoVE Neurosciences
Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

Stereotaxic Injection of a Viral Vector for Conditional Gene Manipulation in the Mouse Spinal Cord

DOI:
10.3791/50313-v

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08:39 min

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March 18, 2013

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, , ,
1Département Nociception et Douleur, Institut des Neurosciences Cellulaires et Intégratives,Centre National de la Recherche Scientifique (CNRS), 2Departments of Anesthesiology and Pharmacology,Columbia University , 3Department of Anesthesiology,Niigata University Graduate School of Medical and Dental Sciences

Chapitres

  • 00:05Titre
  • 01:27Preparation of Equipment and Virus Particle Suspension
  • 03:09Laminectomy
  • 04:57Injection
  • 06:21Wound Closure and Postoperative Care
  • 07:35Results: Transfection of Dorsal Horn Neurons in the Mouse Spinal Cord
  • 08:08Conclusion

Summary

Traduction automatique

Traduction automatique

Viral vectors allow for targeted gene manipulation. We demonstrate a method for conditional gene expression or ablation in the mouse spinal cord, using stereotaxic injection of a viral vector into the dorsal horn, a prominent site of synaptic contact between primary somatosensory afferents and neurons of the central nervous system.

Tags

Gene ManipulationStereotaxic InjectionViral VectorMouse Spinal CordConditional Gene ExpressionGene SilencingDorsal HornLaminectomyAdeno-associated VirusReporter GeneEGFPTransfection

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