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A Simple Method of Mouse Lung Intubation
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Médecine
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Journal JoVE Médecine
A Simple Method of Mouse Lung Intubation

A Simple Method of Mouse Lung Intubation

DOI:
10.3791/50318-v

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07:06 min

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March 21, 2013

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, , ,
1Department of Environmental Health Sciences, Program in Respiratory Biology and Lung Disease,Johns Hopkins Bloomberg School of Public Health, 2Department of Pediatrics,Oregon Health Sciences University

Chapitres

  • 00:05Titre
  • 01:29Preparation
  • 03:17Intubation
  • 05:59Results: Lung Function After Intubation
  • 06:46Conclusion

Summary

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Traduction automatique

This paper describes a striaghforward and efficient method of intubating mice for pulmonary function measurements or pulmonary instillation, that allows the mice to recover and be studied at later times. The procedure involves an inexpensive fiberoptic light source that directly illuminates the trachea.

Tags

Keywords: Mouse Lung IntubationPulmonary Function MeasurementLongitudinal StudyFiberoptic Light SourceLung MechanicsTrachea VisualizationRoutine IntubationStatistical PowerAnimal Minimization

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