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The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells
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Journal JoVE Neurosciences
The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

The Specification of Telencephalic Glutamatergic Neurons from Human Pluripotent Stem Cells

DOI:
10.3791/50321-v

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10:49 min

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April 14, 2013

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, , ,
1Department of Neuroscience,The University of Connecticut Health Center, 2Department of Genetics and Developmental Biology,The University of Connecticut Health Center, 3Stem Cell Institute,The University of Connecticut Health Center

Chapitres

  • 00:05Titre
  • 01:39Generation of Human Pluripotent Stem Cell Aggregates
  • 03:53Induction of Neuroepithelial Cells
  • 05:11Generation of Telencephalic Progenitors
  • 06:33Differentiation into Telencephalic Neurons
  • 08:18Telencephalic Neuronal Generation
  • 10:11Conclusion

Summary

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This procedure yields telencephalic neurons by going through checkpoints which are similar to those observed during human development. The cells are allowed to spontaneously differentiate, are exposed to factors which push them towards the neural lineage, are isolated, and are plated onto coverslips to allow for terminal differentiation and maturation.

Tags

Human Pluripotent Stem CellsTelencephalic Glutamatergic NeuronsNeural InductionNeuroepithelial CellsTelencephalic PrecursorsDorsal Telencephalic ProgenitorsGlutamatergic Neurons

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