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Immunologie et infection
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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
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Immunologie et infection
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Journal JoVE Immunologie et infection
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

DOI:
10.3791/50323-v

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10:07 min

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June 23, 2013

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, , , ,
1Department of Animal Science,Texas A&M University, 2Department of Biology,Texas A&M University, 3Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

Chapitres

  • 00:05Titre
  • 01:36Isolation of Bone Marrow Cells
  • 03:23Induction BMDM Formation
  • 04:17BMDM Polarized Activation
  • 06:11Representative Analysis of BMDM
  • 09:19Conclusion

Summary

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The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

Tags

Macrophage PolarizationBone Marrow Derived MacrophagesGM-CSFM-CSFMonocytic DifferentiationM1 PolarizationM2 PolarizationCell Surface AntigensGene ExpressionCell Signaling Pathways

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