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Neurosciences
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Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

DOI:
10.3791/50326-v

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14:55 min

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May 12, 2013

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,
1Department of Neurology,Washington University in St. Louis School of Medicine

Chapitres

  • 00:05Titre
  • 01:54Pre-Surgical Procedure
  • 03:49Implantation of Alzet Osmotic Pump
  • 08:01Changing or Removing Alzet Osmotic Pump
  • 09:51Administration of an Intracerebroventricular Bolus
  • 12:12Results: Intraventricular Delivery of Antisense Oligonucleotides to the Rodent Central Nervous System
  • 14:36Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a method to target drugs to the central nervous system by either implanting a catheter or performing a bolus injection into the right lateral ventricle in mice. We focus specifically on the delivery of antisense oligonucleotides. This technique is readily adaptable to other drugs and to rats.

Tags

Direct Intraventricular DeliveryCentral Nervous SystemCNSRodentAntisense OligonucleotidesASOsGene KnockdownSplicingAlzet Osmotic PumpsLateral VentricleBolus InjectionCerebral Ventricular System

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