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Måling Intracellulær Ca<sup> 2 +</sup> Endring i Menneskelig Sperm bruker Fire Teknikker: Konvensjonell fluorometri, Stoppet Flow fluorometri, flowcytometrisystemer og Single Cell Imaging
Journal JoVE
Biologie
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Journal JoVE Biologie
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
DOI:

19:26 min

May 24, 2013

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Chapitres

  • 00:05Titre
  • 02:38Sperm Sample Preparation by the Swim-up Method
  • 04:52Fluorescent Dye Loading for Ca2+ Measurements
  • 05:41Conventional Fluorometry
  • 06:59Stopped Flow Fluorometry
  • 08:30Flow Cytometry
  • 09:40Single Cell Imaging
  • 12:11Results: Progesterone Induces Intracellular Ca2+ Changes in Human Sperm Detected by Fluorometric Techniques
  • 17:59Conclusion

Summary

Traduction automatique

Intracellulær Ca<sup> 2 +</sup> Dynamikk er svært viktig i sperm fysiologi og Ca<sup> 2 +</sup>-Sensitive fluorescerende fargestoffer utgjør et allsidig verktøy for å studere dem. Befolkning eksperimenter (fluorometri og stoppet flyt fluorometri) og encellede eksperimenter (flowcytometri og enkelt celle imaging) brukes til å spore spatio-temporal [Ca<sup> 2 +</sup>] Endringer i menneskelige sædceller.

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