Journal
/
Médecine
/
Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

Osmotic Drug Delivery to Ischemic Hindlimbs and Perfusion of Vasculature with Microfil for Micro-Computed Tomography Imaging

DOI:
10.3791/50364-v

•

10:50 min

•

June 29, 2013

•

, , , , , ,
1The Texas Heart Institute at St. Luke’s Episcopal Hospital, 2Department of Vascular Surgery, The Ninth People’s Hospital, School of Medicine,Shanghai Jiao Tong University

Chapitres

  • 00:05Titre
  • 02:47Priming the Osmotic Pump
  • 03:58Creating Unilateral Hindlimb Ischemia in the Mouse
  • 06:30Imaging Specimen Preparation
  • 09:40Results: Representative Perfusion of the Vasculature in the Murine Hindlimb Ischemia Model
  • 10:27Conclusion

Summary

Traduction automatique

Traduction automatique

We show here the in vivo insertion of an osmotic pump for constant local drug delivery and the creation of hindlimb ischemia in a mouse model. Moreover, the hindlimb vasculature is perfused with Microfil, a silicone radiopaque agent, to prepare for micro-computed tomography (micro-CT) imaging.

Tags

Osmotic Drug DeliveryIschemic HindlimbMicrofil PerfusionMicro-computed TomographyPeripheral Arterial DiseasePreclinical ResearchAnimal ModelVascular BiologyAngiogenesisVascular Imaging

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Retrograde Perfusion and Filling of Mouse Coronary Vasculature as Preparation for Micro Computed Tomography Imaging

Assessment of Viability of Human Fat Injection into Nude Mice with Micro-Computed Tomography

Using Micro-computed Tomography for the Assessment of Tumor Development and Follow-up of Response to Treatment in a Mouse Model of Lung Cancer

Intraluminal Drug Delivery to the Mouse Arteriovenous Fistula Endothelium

Combined Near-infrared Fluorescent Imaging and Micro-computed Tomography for Directly Visualizing Cerebral Thromboemboli

Method of Direct Segmental Intra-hepatic Delivery Using a Rat Liver Hilar Clamp Model

Optimized LC-MS/MS Method for the High-throughput Analysis of Clinical Samples of Ivacaftor, Its Major Metabolites, and Lumacaftor in Biological Fluids of Cystic Fibrosis Patients

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells

Tissue Preparation Techniques for Contrast-Enhanced Micro Computed Tomography Imaging of Large Mammalian Cardiac Models with Chronic Disease

DUCT: Double Resin Casting followed by Micro-Computed Tomography for 3D Liver Analysis

Read Article