Journal
/
Biologie
/
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

DOI:
10.3791/50371-v

•

08:05 min

•

June 07, 2013

•

, , , ,
1Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich

Chapitres

  • 00:05Titre
  • 01:09Material Preparation
  • 02:24Seed Dissection and Embryo Extraction
  • 03:49Embryo Isolation
  • 06:23Results: Isolated Plant Embryos Are Suitable for Diverse Downstream Applications
  • 07:36Conclusion

Summary

Traduction automatique

Traduction automatique

We report an efficient and simple method to isolate embryos at early stages of development from Arabidopsis thaliana seeds. Up to 40 embryos can be isolated in 1 hr to 4 hr, depending on the downstream application. The procedure is suitable for transcriptome, DNA methylation, reporter gene expression, immunostaining and fluorescence in situ hybridization analyses.

Tags

Keywords: Arabidopsis ThalianaEarly-stage EmbryosIsolationEndospermSeed IntegumentsSeed CoatManual DissectionGlass MicrocapillaryRT-PCRRNA SequencingDNA MethylationFISHImmunostainingReporter Gene Assays

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50371?article_type=v

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos
check_url/fr/50371?article_type=v

Isolation of Protoplasts from Tissues of 14-day-old Seedlings of Arabidopsis thaliana
check_url/fr/50371?article_type=v

Floral-dip Transformation of Arabidopsis thaliana to Examine pTSO2::β-glucuronidase Reporter Gene Expression
check_url/fr/50371?article_type=v

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
check_url/fr/50371?article_type=v

Isolation and Purification of Kinesin from Drosophila Embryos
check_url/fr/50371?article_type=v

Histochemical Staining of Arabidopsis thaliana Secondary Cell Wall Elements
check_url/fr/50371?article_type=v

Standardized Method for High-throughput Sterilization of Arabidopsis Seeds
check_url/fr/50371?article_type=v

Image-Based Methods to Study Membrane Trafficking Events in Stomatal Lineage Cells (Video) | JoVE

check_url/fr/50371?article_type=v

A High-Resolution, Single-Grain, In Vivo Pollen Hydration Bioassay for Arabidopsis thaliana (Video) | JoVE

check_url/fr/50371?article_type=v

Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae

Read Article