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Effektiv og Rapid Isolering av tidlig stadium embryo fra<em> Arabidopsis thaliana</em> Frø
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Journal JoVE Biologie
Efficient and Rapid Isolation of Early-stage Embryos from Arabidopsis thaliana Seeds

Effektiv og Rapid Isolering av tidlig stadium embryo fra<em> Arabidopsis thaliana</em> Frø

DOI:
10.3791/50371-v

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08:05 min

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June 07, 2013

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, , , ,
1Institute of Plant Biology and Zürich-Basel Plant Science Center,University of Zürich

Chapitres

  • 00:05Titre
  • 01:09Material Preparation
  • 02:24Seed Dissection and Embryo Extraction
  • 03:49Embryo Isolation
  • 06:23Results: Isolated Plant Embryos Are Suitable for Diverse Downstream Applications
  • 07:36Conclusion

Summary

Traduction automatique

Traduction automatique

Vi rapporterer om en effektiv og enkel metode for å isolere embryoer på tidlige stadier av utviklingen fra<em> Arabidopsis thaliana</em> Frø. Opp til 40 embryoene kan bli isolert i 1 time til 4 timer, avhengig av den anvendelse nedstrøms. Fremgangsmåten er egnet for transcriptome, DNA metylering, reporter genekspresjon, farging og fluorescens<em> In situ</em> Hybridiserings-analyser.

Tags

Keywords: Arabidopsis ThalianaEarly-stage EmbryosIsolationEndospermSeed IntegumentsSeed CoatManual DissectionGlass MicrocapillaryRT-PCRRNA SequencingDNA MethylationFISHImmunostainingReporter Gene Assays

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