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Chapitres
- 00:05Titre
- 01:13Inflating the Pancreas
- 03:47Pancreas Removal
- 04:57Washing
- 08:17Picking Islets
- 09:16cAMP Assay
- 11:19Results: Secreted Insulin can be Measured from the cAMP Stimulation Medium
- 12:09Conclusion
Assaying in vitro β-cell function using isolated mouse islets of Langerhans is an important component in the study of diabetes pathophysiology and therapeutics. While many downstream applications are available, this protocol specifically describes the measurement of intracellular cyclic adenosine monophosphate (cAMP) as an essential parameter determining β-cell function.
