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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Journal JoVE
Biologie
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Journal JoVE Biologie
Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae

DOI:
10.3791/50382-v

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07:00 min

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June 14, 2013

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, , , , , ,
1The Lewis-Sigler Institute for Integrative Genomics,Princeton University, 2Graduate Program in Quantitative and Computational Biology,Princeton University, 3Department of Molecular Biology,Princeton University

Chapitres

  • 00:05Titre
  • 01:11Coverslip Preparation
  • 01:51Fixation
  • 03:08Hybridization
  • 04:53Imaging of Cells with Olympus IX-81 Inverted Microscope
  • 05:23Results: Quantitative mRNA Analysis by FISH
  • 06:31Conclusion

Summary

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This protocol describes an experimental procedure for performing Fluorescence in situ Hybridization (FISH) for counting mRNAs in single cells at single-molecule resolution.

Tags

Fluorescence In Situ HybridizationFISHMRNASaccharomyces CerevisiaeSingle-cell AnalysisSingle-moleculeSpot DetectionGene ExpressionVisualizationQuantification

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