Journal
/
Immunologie et infection
/
Isolation, Processing and Analysis of Murine Gingival Cells
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Isolation, Processing and Analysis of Murine Gingival Cells

Isolation, Processing and Analysis of Murine Gingival Cells

DOI:
10.3791/50388-v

•

09:47 min

•

July 02, 2013

•

, , ,
1Institute of Dental Sciences,Hebrew University – Hadassah Medical Center, 2Department of Periodontology,Hebrew University – Hadassah Medical Center

Chapitres

  • 00:05Titre
  • 01:49Upper Gingival Excision
  • 03:24Gingival Processing
  • 04:57Extracellular and Intracellular Antibody Staining for Flow Cytometry Analysis
  • 06:58Representative Gingival Cell Analysis
  • 08:40Conclusion

Summary

Traduction automatique

Traduction automatique

This study describes an efficient technique to isolate and process gingival tissues from the mouse oral cavity in order to produce a single-cell culture. The resulting cells can be further used for flow cytometry analysis and molecular studies.

Tags

Keywords: Murine Gingival CellsIsolationFlow CytometryMolecular StudiesImmune MechanismsPeriodontal DiseasesAlveolar BoneLangerhans CellsT Regulatory CellsOral Immune System

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50388?article_type=v

Flow Cytometry Analysis of Immune Cells Within Murine Aortas
check_url/fr/50388?article_type=v

Isolation, Identification, and Purification of Murine Thymic Epithelial Cells
check_url/fr/50388?article_type=v

Isolation of Murine Lymph Node Stromal Cells
check_url/fr/50388?article_type=v

Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
check_url/fr/50388?article_type=v

Isolation and Flow Cytometric Analysis of Immune Cells from the Ischemic Mouse Brain
check_url/fr/50388?article_type=v

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
check_url/fr/50388?article_type=v

Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
check_url/fr/50388?article_type=v

Isolation and Activation of Murine Lymphocytes
check_url/fr/50388?article_type=v

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
check_url/fr/50388?article_type=v

Digestion of the Murine Liver for a Flow Cytometric Analysis of Lymphatic Endothelial Cells

Read Article